Healthcare-associated infections (HAIs) are a major patient safety concern for healthcare organizations. The Centers for Disease Control and Prevention estimates that on any given day nearly 1 out of every 25 inpatients in U.S. acute care hospitals have at least one HAI. Pneumonia, surgical site, and device-associated infections (i.e., central catheter-associated bloodstream infection, catheter-associated urinary tract infection, ventilator-associated pneumonia) are the most common HAIs. Clostridioides difficile and methicillin-resistant Staphylococcus aureus (MRSA) are the most common pathogens. HAIs result in more than 75,000 deaths annually and add billions of dollars to the cost of patient care. Healthcare workers are at risk for acquiring infections from patients and other healthcare workers. An effective infection prevention program that includes education and the use of infection prevention practices by healthcare workers can reduce the risk of acquiring and transmitting infections.